Key Product Details

Species Reactivity

Human

Applications

ELISA, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 61016.11

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all CCL24/Eotaxin-2/MPIF-2 Antibodies »

Product Specifications

Immunogen

Purified recombinant human eotaxin 2, expressed in E. coli as immunogen.

Localization

Secreted

Specificity

When used as a capture in human eotaxin 2 sandwich ELISAs, this exhibits less than 0.03 % cross-reactivity with recombinant human eotaxin, MIP1 alpha, and MCP3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use at a concentration of 2 ug/ml. Neut: Use at a concentration of 1 - 5 ug/ml. WB: Use at a concentration of 1 - 2 ug/ml. Predicted molecular weight: 10.6 kDa.

Neutralization: antibody may be used for neutralization assays at a working concentration of 1-5 ug/ml in the presence of 100 ng/ml of human recombinant eotaxin-2.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.

Alternate Names

Eotaxin-2, MPIF-2, MPIF2

Entrez Gene IDs

6369 (Human)

Gene Symbol

CCL24

Additional CCL24/Eotaxin-2/MPIF-2 Products

Product Documents for CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCL24/Eotaxin-2/MPIF-2 Antibody (61016.11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.