CCL4/MIP-1 beta Antibody (7C9E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27128

Novus Biologicals, part of Bio-Techne
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NBP3-27128-0.025ml
NBP3-27128-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7C9E4

Concentration

1 mg/ml

View all CCL4/MIP-1 beta Antibodies »

Product Summary for CCL4/MIP-1 beta Antibody (7C9E4)

Immunogen

Purified recombinant fragment of human CCL4/MIP-1 beta (AA: 24-92) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

10.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CCL4/MIP-1 beta Antibody (7C9E4)

ELISA: CCL4/MIP-1 beta Antibody (7C9E4) [NBP3-27128] -

ELISA: CCL4/MIP-1 beta Antibody (7C9E4) [NBP3-27128] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: CCL4/MIP-1 beta Antibody (7C9E4) [NBP3-27128] -

Western Blot: CCL4/MIP-1 beta Antibody (7C9E4) [NBP3-27128] - Analysis using CCL4/MIP-1 beta mAb against HEK293 (1) and CCL4/MIP-1 beta (AA: 24-92)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: CCL4/MIP-1 beta Antibody (7C9E4) [NBP3-27128] -

Flow Cytometry: CCL4/MIP-1 beta Antibody (7C9E4) [NBP3-27128] - Analysis of HL-60 cells using CCL4/MIP-1 beta mouse mAb (green) and negative control (red).
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Applications for CCL4/MIP-1 beta Antibody (7C9E4)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL4/MIP-1 beta

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

Alternate Names

Exodus-3, MIP-1 beta, MIP1 beta

Gene Symbol

CCL4

Additional CCL4/MIP-1 beta Products

Product Documents for CCL4/MIP-1 beta Antibody (7C9E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CCL4/MIP-1 beta Antibody (7C9E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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