CCL4/MIP-1 beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77897

Novus Biologicals, part of Bio-Techne
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NBP1-77897
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Key Product Details

Species Reactivity

Validated:

Porcine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CCL4/MIP-1 beta Antibodies »

Product Summary for CCL4/MIP-1 beta Antibody

Immunogen

This protein-A purified CCL4/MIP-1 beta Antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP-1 beta protein. (Uniprot: Q711P4)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with CCL4/MIP-1 beta from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4/MIP-1 beta based on 90% homology. Cross-reactivity with CCL4/MIP-1 beta from other sources has not been determined. The swine CCL4/MIP-1 beta sequence is also 86% homologous to swine CCL3L1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was Protein-A purified from monospecific antiserum by chromatography

Store vial at 4C upon receipt. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for CCL4/MIP-1 beta Antibody

Western Blot: CCL4/MIP-1 beta Antibody [NBP1-77897]

Western Blot: CCL4/MIP-1 beta Antibody [NBP1-77897]

Western Blot: CCL4L1/LAG-1 Antibody [NBP1-77897] - Shows detection of recombinant swine MIP-1b (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 (TM) conjugated Goat Anti-Rabbit IgG at 1:20,000 for 45 min at room temperature.

Applications for CCL4/MIP-1 beta Antibody

Application
Recommended Usage

ELISA

1:5000

Western Blot

1:500
Application Notes
This protein-A purified This product has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL4/MIP-1 beta

In many species, both C-C chemokines macrophage inflammatory protein, or MIP-1 alpha (CCL3L1), and MIP-1 beta (CCL4) are structurally and functionally related CC chemokines. They are both potent chemoattractants for monocytes, which form an important component of the stroma of tumor tissue, and in humans may regulate tumor growth and associated inflammation. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. Both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes, but their effect on lymphocytes differ. MIP-1 alpha selectively attracts CD8+ lymphocytes, while MIP-1 beta selectively attracts CD4+ lymphocytes. They contain the four highly conserved cysteine residues present in CC chemokines. MIP-1 beta (CCL4) has specificity for CCR5 receptors. In humans, it is also a major HIV-suppressive factor produced by CD8+ T cells.

Alternate Names

Exodus-3, MIP-1 beta, MIP1 beta, MIP-1 beta/CCL4

Gene Symbol

CCL4

UniProt

Q711P4 (Porcine)

Additional CCL4/MIP-1 beta Products

Product Documents for CCL4/MIP-1 beta Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CCL4/MIP-1 beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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