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CCL8/MCP-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-79937

Novus Biologicals, part of Bio-Techne
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NBP1-79937

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide directed towards the middle region of human CCL8. Peptide sequence SYTRITNIQCPKEAVIFKTKRGKEVCADPKERWVRDSMKHLDQIFQNLKP. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for CCL8/MCP-2 Antibody

Western Blot: CCL8/MCP-2 Antibody [NBP1-79937]

Western Blot: CCL8/MCP-2 Antibody [NBP1-79937]

Western Blot: CCL8/MCP-2 Antibody [NBP1-79937] - Host: Rabbit. Target: CCL8. Positive control (+): Human Fetal Heart (HE). Negative control (-): HeLa Cell Lysate (HL). Antibody concentration: 1ug/ml
Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody [NBP1-79937]

Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody [NBP1-79937]

Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody [NBP1-79937] - Rabbit Anti-CCL8 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adrenal. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x. Exposure Time: 0.5-2.0sec
Western Blot: CCL8/MCP-2 Antibody [NBP1-79937]

Western Blot: CCL8/MCP-2 Antibody [NBP1-79937]

Western Blot: CCL8/MCP-2 Antibody [NBP1-79937] - Human Heart lysate, concentration 0.2-1 ug/ml.

Applications for CCL8/MCP-2 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL8/MCP-2

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Gene Symbol

CCL8

Additional CCL8/MCP-2 Products

Product Documents for CCL8/MCP-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCL8/MCP-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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