CCM2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-53812
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KGEKSRDKKAHEK, from the internal region of the protein sequence according to NP_001025006.1; NP_113631.1; NP_001161407.1; NP_001350387.1.
Specificity
This antibody is expected to recognize isoform 1 (NP_001025006.1), isoform 2 (NP_113631.1) and isoform 4 (NP_001161407.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for CCM2 Antibody
Flow Cytometry: CCM2 Antibody [NB100-53812] -
Flow Cytometry: CCM2 Antibody [NB100-53812] - Flow cytometric analysis of paraformaldehyde fixed KNRK cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: CCM2 Antibody [NB100-53812] -
Western Blot: CCM2 Antibody [NB100-53812] - (1ug/ml) staining of Rat KNRK cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: CCM2 Antibody [NB100-53812] -
Immunocytochemistry/Immunofluorescence: CCM2 Antibody [NB100-53812] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for CCM2 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
0.3-2 ug/ml
Application Notes
WB: Approx 45-48kDa band observed in Human Heart lysates and approx.48kDa in Human Cerebral Cortex lysates and in lysates of cell line KNRK (calculated MW of 48.8kDa according to Human NP_113631.1 and 49.8kDa according to Rat NP_001119747.1). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CCM2
Alternate Names
C7orf22OSM, cerebral cavernous malformation 2, Cerebral cavernous malformations 2 protein, chromosome 7 open reading frame 22, malcavernin, MGC4067, MGC4607, MGC74868
Gene Symbol
CCM2
UniProt
Additional CCM2 Products
Product Documents for CCM2 Antibody
Product Specific Notices for CCM2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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