CCR5 Antibody (12D1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47759

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 12D1

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CCR5 Antibodies »

Product Specifications

Immunogen

Human native CCR5 protein (Uniprot: P51681)

Localization

Cell surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44483).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CCR5 Antibody (12D1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759]

Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759]

Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).
Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759]

Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759]

Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Human Stomach tissue stained with CCR5 Antibody (12D1).
Flow Cytometry: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759]

Flow Cytometry: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759]

Flow Cytometry: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Flow Cytometric Analysis of MCF-7 cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for CCR5 Antibody (12D1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Frozen

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CCR5

C-C chemokine receptor type 5 (CCR5) is a member of the beta chemokine receptors family of integral membrane proteins. The CCR5 protein is a G protein-coupled receptor which functions as a chemokine receptor in the CC chemokine group.

CCR5 is a major coreceptor for human HIV infection and is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.

CCR5 antibodies are useful for HIV infection studies and research on immune responses.

Alternate Names

CCR5, CD195

Gene Symbol

CCR5

Additional CCR5 Products

Product Documents for CCR5 Antibody (12D1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CCR5 Antibody (12D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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