CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12000
Recombinant Monoclonal Antibody
![Western Blot: CCR5 Antibody (RC-10)ChimericAzide and BSA Free [NBP3-12000]](https://resources.bio-techne.com/images/products/CCR5-Antibody-RC-10-Chimeric-Western-Blot-NBP3-12000-img0001.jpg "Western Blot: CCR5 Antibody (RC-10)ChimericAzide and BSA Free [NBP3-12000]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # RC-10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing mice with CCR5.
Specificity
This antibody recognizes non-activated nonphospho-CCR5. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free
Western Blot: CCR5 Antibody (RC-10)ChimericAzide and BSA Free [NBP3-12000]
Western Blot: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Jurkat (A), Molt4 (B), K562 (C) and Daudi (D) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RC-10 (NBP3-12000) at 0.0003 ug/ml, 0.001 ug/ml, 0.003 ug/ml and unknown ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free [NBP3-12000]](https://resources.bio-techne.com/images/products/CCR5-Antibody-RC-10-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12000-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free [NBP3-12000]")
Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free [NBP3-12000]
Immunocytochemistry/Immunofluorescence: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RC-10 (NBP3-12000) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12000, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CCR5
CCR5 is a major coreceptor for human HIV infection and is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.
CCR5 antibodies are useful for HIV infection studies and research on immune responses.
Alternate Names
CCR5, CD195
Gene Symbol
CCR5
Additional CCR5 Products
Product Documents for CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free
Product Specific Notices for CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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