CCR5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31374
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Monkey
Cited:
Human, Mouse
Predicted:
Primate (99%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an N-terminal portion of the human CCR5 protein (between amino acids 1-350)
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 21305529). Rhesus macaque reactivity reported in a verified customer review.
Localization
Multi-pass membrane protein with extra-cellular and cytoplasmic domains
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CCR5 Antibody
Western Blot: CCR5 Antibody [NBP2-31374]
Western Blot: CCR5 Antibody [NBP2-31374] - Image of anti-CCR5 antibody. Whole cell protein from Daudi (1), K562 (2) and THP-1 (3) cell lines were separated by SDS-PAGE and protein transfered to PVDF. The membrane was probed with anti-CCR5 antibody at 2 ug/mL and detected with an anti-rabbit-HRP secondary antibody and chemiluminescence. CCR5 is shown at 40 kDa.Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374]
Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Detection of CCR5 and virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were uninfected (A) and untreated controls, and Meth-only treated (B). Pictures were at 16x magnification. Sites of interest (rectangles) were further magnified. Image collected and cropped by CiteAb from the following publication (https://bmcimmunol.biomedcentral.com/articles/10.1186/s12865-016-0145-0), licensed under a CC-BY license.Western Blot: CCR5 Antibody [NBP2-31374]
Western Blot: CCR5 Antibody [NBP2-31374] - Detection of CCR5 partial recombinant protein by using CCR5 antibody at a concentration of 0.5 ug/mL.Applications for CCR5 Antibody
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Simple Western
1:50
Western Blot
2 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP2-31374 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCR5
Additional CCR5 Products
Product Documents for CCR5 Antibody
Product Specific Notices for CCR5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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