Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 18B9E6

Format

BSA Free

Concentration

1.0 mg/ml

View all CCR6 Antibodies »

Product Specifications

Immunogen

A recombinant protein consisting of the external domains of mouse CD196 (CCR6) was used as the immunogen for the antibody.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28684423).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for CCR6 Antibody (18B9E6) - BSA Free

Western Blot: CCR6 Antibody (18B9E6)BSA Free [NBP2-25220]

Western Blot: CCR6 Antibody (18B9E6)BSA Free [NBP2-25220]

Western Blot: CCR6 Antibody (18B9E6) [NBP2-25220] - A) mouse EL4, B) conA treated EL4 lysate and C) partial recombinant protein using CD196 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - BSA Free [NBP2-25220]

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - BSA Free [NBP2-25220]

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - BSA Free [NBP2-25220] - CCR6 staining using GE Cell DIVE (20x) on Human Rheumatoid Arthritis synovium (10 ug/ml w/ 1:500 Donkey anti-Rat AF555). Image from verified customer review.
Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - BSA Free [NBP2-25220]

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - BSA Free [NBP2-25220]

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) [NBP2-25220] - Normal mouse testis tissue stained with CD196 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining.

Applications for CCR6 Antibody (18B9E6) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reviewed Applications

Read 1 review rated 3 using NBP2-25220 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCR6

CD196 (CCR6) is a chemokine receptor expressed on Th17 cells and regulatory Tcells that is induced by T-cell priming with certain cytokines. This protein plays a pivotal role in directing inflammatory and regulatory cells to inflamed tissues or the gut, where the ligand of CD196, CCL20 is expressed. Although most chemokine-chemokine receptor interactions are characterized by a high promiscuity, CD196 binds only CCL20 and -defensins. This chemokine receptor is widely expressed on human blood and tissue leukocytes, including subsets of dendritic cells, CD45RO+ effector/memory Tcells, CD25 high regulatory Tcells (Tregs), naive and memory B cells, NKT cells, and NK cells. On memory Tcells, CD196 is expressed on a population of autoreactive interleukin-10 (IL-10)-producing cells and subsets of skin- and mucosa-homing cells. More recently, CD196 was identified as a new risk factor for rheumatoid arthritis; moreover, a nucleotide polymorphism within the Cd196locus was shown to be associated with susceptibility to rheumatoid arthritis, Graves disease, and Crohn disease (Steinfelder S et al, .2011).This protein also acts as a marker for Th17 lineage polarization and HIV permissiveness in memory CD4+ Tcells. CD196+ Tcells have the potential to migrate into the GALT via the gut-homing integrin 47, a newly identified HIV-gp120 binding receptor. Nevertheless, given the ability of integrin 7 to regulate cell migration into the GALT and bind HIV-gp120, CD196+ Tcells coexpressing integrin 7 and CCR5 might have an extraordinary ability to disseminate HIV from the portal sites of entry (Monteiro P et al ,. 2011). Anti-CD196 antibody could almost completely suppress the cell migration induced by hBD-2 (Human beta-defensin-2), suggesting that hBD-2 mainly utilizes CD196 (CCR6) as a functional receptor (Niyonsaba F et al,.2004).

Alternate Names

CC-CKR-6, CCR6, CD196, CKR-L3, CKRL3, CMKBR6, DRY6, GPR-CY4, GPR29, GPRCY4, STRL22

Gene Symbol

CCR6

UniProt

NP_001177262 (Mouse)

Additional CCR6 Products

Product Documents for CCR6 Antibody (18B9E6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CCR6 Antibody (18B9E6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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