Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 18B9E6

Format

Azide Free

Concentration

1.0 mg/ml

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Product Summary for CCR6 Antibody (18B9E6) - Azide Free

Immunogen

A recombinant protein consisting of the external domains of mouse CD196 (CCR6) was used as the immunogen for the antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for CCR6 Antibody (18B9E6) - Azide Free

Western Blot: CCR6 Antibody (18B9E6)Azide Free [NBP2-25224]

Western Blot: CCR6 Antibody (18B9E6)Azide Free [NBP2-25224]

Western Blot: CCR6 Antibody (18B9E6) - Azide Free [NBP2-25224] - analysis of A) mouse EL4, B) conA treated EL4 lysate and C) partial recombinant protein using CD196 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - Azide Free [NBP2-25224]

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - Azide Free [NBP2-25224]

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - Azide Free [NBP2-25224] - Formalin-fixed, paraffin-embedded normal mouse testis tissue stained with CD196 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining.

Applications for CCR6 Antibody (18B9E6) - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Sterile PBS

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCR6

CD196 (CCR6) belongs to family A of the G protein-coupled receptor superfamily. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dendritic and T cells during inflammatory and immunological responses.

Alternate Names

CC-CKR-6, CCR6, CD196, CKR-L3, CKRL3, CMKBR6, DRY6, GPR-CY4, GPR29, GPRCY4, STRL22

Gene Symbol

CCR6

Additional CCR6 Products

Product Documents for CCR6 Antibody (18B9E6) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CCR6 Antibody (18B9E6) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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