CCR8 Antibody (9N1Y1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16370

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16370-100ul
NBP3-16370-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9N1Y1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CCR8 Antibodies »

Product Summary for CCR8 Antibody (9N1Y1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-355 of human CCR8 (P51685). FWVPFNVVLFLTSLHSMHILDGCSISQQLTYATHVTEIISFTHCCVNPVIYAFVGEKFKKHLSEIFQKSCSQIFNYLGRQMPRESCEKSSSCQQHSSRSSSVDYIL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CCR8 Antibody (9N1Y1)

Western Blot: CCR8 Antibody (9N1Y1) [NBP3-16370]

Western Blot: CCR8 Antibody (9N1Y1) [NBP3-16370]

Western Blot: CCR8 Antibody (9N1Y1) [NBP3-16370] - Western blot analysis of extracts of various cell lines, using CCR8 Rabbit mAb (NBP3-16370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: CCR8 Antibody (9N1Y1) [NBP3-16370]

Immunocytochemistry/ Immunofluorescence: CCR8 Antibody (9N1Y1) [NBP3-16370]

Immunocytochemistry/Immunofluorescence: CCR8 Antibody (9N1Y1) [NBP3-16370] - Immunofluorescence analysis of Jurkat cells using CCR8 Rabbit mAb (NBP3-16370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for CCR8 Antibody (9N1Y1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCR8

CKR8/CCR8, or C-C chemokine receptor type 8, is a G protein-coupled, seven-transmembrane domain receptor protein. Chemokines are key molecules in directing leukocyte migration toward sites of inflammation. The human C-C chemokine I-309 is a monocyte chemoattractant and inhibits apoptosis in thymic cell lines. It has been found that CKR8/CCR8 is a receptor for chemokines SCYA1/I-309, SCYA4/MIP-1-beta, and SCYA17/TARC (1-3).

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Gene Symbol

CCR8

Additional CCR8 Products

Product Documents for CCR8 Antibody (9N1Y1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CCR8 Antibody (9N1Y1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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