CD117/c-kit Antibody (CL1657)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52975

Novus Biologicals, part of Bio-Techne
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NBP2-52975
NBP2-52975-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1657

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQ

Epitope

ILDETNENKQNEWIT

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD117/c-kit Antibody (CL1657)

Western Blot: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Western Blot: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Western Blot: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human stomach shows strong positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human cerebellum shows moderate positivity in neuropil of the molecular layer.
View 3 more images

Applications for CD117/c-kit Antibody (CL1657)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (CL1657)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD117/c-kit Antibody (CL1657)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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