CD117/c-kit [p Tyr719] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16899

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-16899-100ul
NBP3-16899-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y719 of mouse CD117/c-kit (NP_066922.2). TNEYM

Modification

p Tyr719

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD117/c-kit [p Tyr719] Antibody - BSA Free

Western Blot: CD117/c-kit [p Tyr719] AntibodyBSA Free [NBP3-16899]

Western Blot: CD117/c-kit [p Tyr719] AntibodyBSA Free [NBP3-16899]

Western Blot: CD117/c-kit [p Tyr719] Antibody [NBP3-16899] - Analysis of extracts of NIH/3T3 cells, using CD117/c-kit antibody (NBP3-16899) at 1:500 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for CD117/c-kit [p Tyr719] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit [p Tyr719] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD117/c-kit [p Tyr719] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.