CD11b Antibody (M1/70.15) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80626
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rabbit
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Lambda Clone # M1/70.15
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD11b Antibody (M1/70.15) - Azide and BSA Free
Immunogen
Rat Monoclonal CD11b Antibody (M1/70.15) was made to T cell enriched splenocytes from B10 mice.
Reactivity Notes
Mouse reactivity reported in a verified customer review. Rabbit reactivity reported in scientific literature (PMID: 28700779). Human reactivity reported in scientific literature (PMID: 28683563).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Lambda
Theoretical MW
127.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD11b Antibody (M1/70.15) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]
Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red). Image from the standard format of this antibody.![Immunohistochemistry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80626-img0005.jpg "Immunohistochemistry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
Immunohistochemistry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]
Immunohistochemistry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody. Image from the standard format of this antibody.![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0008.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]
Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0006.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0007.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]](https://resources.bio-techne.com/images/products/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0009.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626]")
Applications for CD11b Antibody (M1/70.15) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:50. Use reported in scientific literature (PMID 28683563)
Immunocytochemistry/ Immunofluorescence
1:10-1:500. Use reported by customer review
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500. Use reported in scientific literature
Immunohistochemistry-Paraffin
1:10-1:500. Use reported in scientific literature (PMID 28700779)
Immunoprecipitation
1:10-1:500
Application Notes
IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. NB600-1327 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD11b/Integrin alpha M
References
1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373
2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677
3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593
4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
Additional CD11b/Integrin alpha M Products
Product Documents for CD11b Antibody (M1/70.15) - Azide and BSA Free
Product Specific Notices for CD11b Antibody (M1/70.15) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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