I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?

Name: CD11b Antibody (M1/70.15) - BSA Free
Brand: Novus Biologicals
SKU: NB600-1327
Rating: 5 (2 reviews)

I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.

CD11b Antibody (M1/70.15) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1327

(2)

Citations (18)

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB600-1327AF350

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Alexa Fluor 405

NB600-1327AF405

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Alexa Fluor 488

NB600-1327AF488

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Alexa Fluor 532

NB600-1327AF532

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Alexa Fluor 594

NB600-1327AF594

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Alexa Fluor 647

NB600-1327AF647

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Alexa Fluor 700

NB600-1327AF700

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Alexa Fluor 750

NB600-1327AF750

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Allophycocyanin

NB600-1327APC

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Allophycocyanin/Cy7

NB600-1327APCCY7

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Biotin

NB600-1327B

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CoraFluor 1

NB600-1327CL1

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DyLight 350

NB600-1327UV

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DyLight 405

NB600-1327V

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DyLight 488

NB600-1327G

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DyLight 550

NB600-1327R

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DyLight 594

NB600-1327DL594

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DyLight 650

NB600-1327C

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DyLight 680

NB600-1327FR

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DyLight 755

NB600-1327IR

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FITC

NB600-1327F

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HRP

NB600-1327H

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Janelia Fluor 525

NB600-1327JF525

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Janelia Fluor 549

NB600-1327JF549

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Janelia Fluor 585

NB600-1327JF585

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Janelia Fluor 635

NB600-1327JF635

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Janelia Fluor 646

NB600-1327JF646

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Janelia Fluor 669

NB600-1327JF669

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mFluor Violet 450 SE

NB600-1327MFV450

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mFluor Violet 500 SE

NB600-1327MFV500

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mFluor Violet 610 SE

NB600-1327MFV610

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NB600-1327PE

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PE/Atto594

NB600-1327PEATT594

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PE/Cy5.5

NB600-1327PECY55

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PE/Cy7

NB600-1327PECY7

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PerCP

NB600-1327PCP

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Forumulation

Catalog #

Azide and BSA Free

NBP2-80626

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100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Lambda Clone # M1/70.15

Format

BSA Free

Concentration

1.0 mg/ml

View all CD11b/Integrin alpha M Antibodies »

Product Specifications

Immunogen

Rat Monoclonal CD11b Antibody (M1/70.15) was made to T cell enriched splenocytes from B10 mice.

Reactivity Notes

Mouse reactivity reported in a verified customer review. Rabbit reactivity reported in scientific literature (PMID: 28700779). Human reactivity reported in scientific literature (PMID: 28683563).

Marker

Microglia Marker, Myeloid Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Lambda

Theoretical MW

127.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD11b Antibody (M1/70.15) - BSA Free

Immunohistochemistry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear blue due to 4',6-diamidino-2-phenylindole (DAPI) counter staining.

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to DyLight 550. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature.

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] (NB600-1327) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 488 (NB600-1327AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red).

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) [NB600-1327]. Image from a verified customer review.

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with lysophosphatidic acid (LPA) or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b Antibody (M1/70.15) [NB600-1327] was used in a dilution 1:100 in order to stain primary murine microglia. Cy2 (green) and Cy3 (red) were used as secondary antibodies.

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 647 (NB600-1327AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327].

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].

CD11b (M1/70.15) in Raw 264.7 Mouse Cell Line.

CD11b (M1/70.15) was detected in immersion fixed Raw 264.7 mouse macrophage cell line using Rat anti-CD11b (M1/70.15) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NB600-1327R) (red) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

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Applications for CD11b Antibody (M1/70.15) - BSA Free

Application

Recommended Usage

Flow Cytometry

1:50. Use reported in scientific literature (PMID 28683563)

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported by customer review

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500. Use reported in scientific literature

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (PMID 28700779)

Immunoprecipitation

1:10-1:500

Application Notes

IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. NB600-1327 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This antibody is CyTOF ready.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB600-1327 in the following applications:

Immunocytochemistry (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD11b/Integrin alpha M

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). The CD11b/CD18 (Mac-1) heterodimer has a theoretical molecular weight of 127kDa but is often observed near 170kDa due to post translational modifications. Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix. Integrins have diverse roles in several biological processes, including cell migration during development, wound healing, cell differentiation, and apoptosis. The particular integrin CD11b is known as a pro-inflammatory molecule given its ability to promote phagocyte cytotoxic functions while enhancing several effector molecules such as FcGR, uPAR, and CD14 (1). In macrophages, CpG-DNA stimulates lysosomal degradation and vacuolar acidification, subsequently promoting CD11b release via TLR9 (2). During an acute infection, high CD11b/Mac-1 expression on antigen-specific CD8 (+) T cells can signify recent activation of the immune system, whereas naive T cells and virus-specific memory CD8 (+) T cells express little or no Mac-1 when exposed to a virus (3). Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder (4).

References

1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373

2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677

3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593

4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71

Alternate Names

CD11b, Integrin alpha M, ITGAM, cd11b flow antibody, M1/70, M1/70 cd11b, m1/70 clone, M1/70.15 Antibody, M1/70.15 CD11b, M1/70.15 CD11b Antibody, M1/70.15 Clone, M1/70.15 Conjugated, M1/70.15 ICC Antibody, M1/70.15 IHC Antibody, M1/70.15 Immunocytochemistry Antibody, M1/70.15 Immunohistochemistry Antibody, M1/70.15 Microglia Marker, M1/70.15 Myeloid Marker

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

UniProt

P05555 (Mouse)

Additional CD11b/Integrin alpha M Products

Product Documents for CD11b Antibody (M1/70.15) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD11b Antibody (M1/70.15) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD11b Antibody (M1/70.15) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1327

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all CD11b/Integrin alpha M Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Marker

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for CD11b Antibody (M1/70.15) - BSA Free

Immunohistochemistry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

Flow Cytometry: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327]

CD11b (M1/70.15) in Raw 264.7 Mouse Cell Line.

Applications for CD11b Antibody (M1/70.15) - BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD11b/Integrin alpha M

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CD11b/Integrin alpha M Products

Product Documents for CD11b Antibody (M1/70.15) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD11b Antibody (M1/70.15) - BSA Free