CD11b Antibody (PSH03-96)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32121

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32121
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH03-96

Concentration

1 mg/ml

View all CD11b/Integrin alpha M Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CD11b aa 1-100. (Uniprot: P11215)

Localization

Cell membrane, Membrane raft.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD11b Antibody (PSH03-96)

CD11b Antibody (PSH03-96)

Western Blot: CD11b Antibody (PSH03-96) [NBP3-32121]

Western Blot: CD11b Antibody (PSH03-96) [NBP3-32121] - Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (NBP3-32121) at 1/2,000 dilution.

Lane 1: TF-1 cell lysate (10 ug/Lane)
Lane 2: THP-1 cell lysate (15 ug/Lane)
Lane 3: U-937 cell lysate (30 ug/Lane)
Lane 4: Jurkat cell lysate (negative) (10 ug/Lane)
Lane 5: RAW264.7 cell lysate (5 ug/Lane)
Lane 6: J774A.1 cell lysate (5 ug/Lane)

Predicted band size: 127 kDa
Observed band size: 170 kDa

Exposure time: 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
CD11b Antibody (PSH03-96)

Immunohistochemistry: CD11b Antibody (PSH03-96) [NBP3-32121]

Immunohistochemistry: CD11b Antibody (PSH03-96) [NBP3-32121] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (NBP3-32121) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32121) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for CD11b Antibody (PSH03-96)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:5000-1:20000

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD11b/Integrin alpha M

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). The CD11b/CD18 (Mac-1) heterodimer has a theoretical molecular weight of 127kDa but is often observed near 170kDa due to post translational modifications. Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix. Integrins have diverse roles in several biological processes, including cell migration during development, wound healing, cell differentiation, and apoptosis. The particular integrin CD11b is known as a pro-inflammatory molecule given its ability to promote phagocyte cytotoxic functions while enhancing several effector molecules such as FcGR, uPAR, and CD14 (1). In macrophages, CpG-DNA stimulates lysosomal degradation and vacuolar acidification, subsequently promoting CD11b release via TLR9 (2). During an acute infection, high CD11b/Mac-1 expression on antigen-specific CD8 (+) T cells can signify recent activation of the immune system, whereas naive T cells and virus-specific memory CD8 (+) T cells express little or no Mac-1 when exposed to a virus (3). Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder (4).

References

1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373

2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677

3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593

4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71

Alternate Names

CD11b, Integrin alpha M, ITGAM

Gene Symbol

ITGAM

Additional CD11b/Integrin alpha M Products

Product Documents for CD11b Antibody (PSH03-96)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD11b Antibody (PSH03-96)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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