CD11b Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-89474
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque
Cited:
Human, Mouse, Rat, Bovine, Hamster - Cricetulus (Chinese Hamster), Nematode - Caenorhabditis elegans, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Dual RNAscope ISH-IHC, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In-situ Hybridization, Simple Western, Single Cell Western, Western Blot
Cited:
Block/Neutralize, Dual RNAscope ISH-IHC, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Rabbit Polyclonal CD11b Antibody was made to a synthetic peptide within residues 250-350 of the mouse CD11b protein. [Swiss-Prot# P05555]
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:35398596).Monkey reactivity reported in scientific literature (PMID: 26443820). Rhesus Macaque reactivity reported in scientific literature (PMID: 29760177). Use in C. elegans reported in scientific literature (PMID:32058942).
Localization
Monocytes and granulocytes.
Marker
Microglia Marker, Myeloid Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
127.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD11b Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474]
Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody (NB110-89474) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Immunocytochemistry/ Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474]
Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Western Blot: CD11b AntibodyBSA Free [NB110-89474]
Western Blot: CD11b Antibody - BSA Free [NB110-89474] - Western Blot detection of CD11b in RAW 264.7 whole cell lysates using [NB110-89474].Applications for CD11b Antibody - BSA Free
Application
Recommended Usage
Flow (Cell Surface)
1:10 - 1:1000
Flow Cytometry
reported in scientific literature (PMID 21422470)
Immunocytochemistry/ Immunofluorescence
1:200. Use reported in multiple pieces of scientific literature (PMID 23980916)
Immunohistochemistry
1:400
Immunohistochemistry-Frozen
reported in scientific literature (PMID 23980916)
Immunohistochemistry-Paraffin
1:400. Use reported in scientific literature (PMID 31022918)
In-situ Hybridization
reported in scientific literature (PMID 27133471)
Simple Western
1:50
Single Cell Western
1:10
Western Blot
2 ug/mL. Use reported in scientific literature (PMID 31082627)
Application Notes
In WB a specific band is observed ~160 kDa and an apparant non-specific band is observed ~56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human samples with WB. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 12 reviews rated 4.5 using NB110-89474 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CD11b/Integrin alpha M
References
1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373
2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677
3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593
4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71
Alternate Names
CD11b, Integrin alpha M, ITGAM, MAC 1, MAC1, Mac1, alpha subunit, Mac-1a, Mo1, alpha subunit
Gene Symbol
ITGAM
UniProt
Additional CD11b/Integrin alpha M Products
Product Documents for CD11b Antibody - BSA Free
Product Specific Notices for CD11b Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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