CD11b Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-89474

Novus Biologicals, part of Bio-Techne
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NB110-89474
NB110-89474SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque

Cited:

Human, Mouse, Rat, Bovine, Hamster - Cricetulus (Chinese Hamster), Nematode - Caenorhabditis elegans, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Dual RNAscope ISH-IHC, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In-situ Hybridization, Simple Western, Single Cell Western, Western Blot

Cited:

Block/Neutralize, Dual RNAscope ISH-IHC, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CD11b/Integrin alpha M Antibodies »

Product Specifications

Immunogen

Rabbit Polyclonal CD11b Antibody was made to a synthetic peptide within residues 250-350 of the mouse CD11b protein. [Swiss-Prot# P05555]

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35398596).Monkey reactivity reported in scientific literature (PMID: 26443820). Rhesus Macaque reactivity reported in scientific literature (PMID: 29760177). Use in C. elegans reported in scientific literature (PMID:32058942).

Localization

Monocytes and granulocytes.

Marker

Microglia Marker, Myeloid Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

127.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD11b Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474]

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody (NB110-89474) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474]

Immunocytochemistry/ Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474]

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: CD11b AntibodyBSA Free [NB110-89474]

Western Blot: CD11b AntibodyBSA Free [NB110-89474]

Western Blot: CD11b Antibody - BSA Free [NB110-89474] - Western Blot detection of CD11b in RAW 264.7 whole cell lysates using [NB110-89474].
View 21 more images

Applications for CD11b Antibody - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1:10 - 1:1000

Flow Cytometry

reported in scientific literature (PMID 21422470)

Immunocytochemistry/ Immunofluorescence

1:200. Use reported in multiple pieces of scientific literature (PMID 23980916)

Immunohistochemistry

1:400

Immunohistochemistry-Frozen

reported in scientific literature (PMID 23980916)

Immunohistochemistry-Paraffin

1:400. Use reported in scientific literature (PMID 31022918)

In-situ Hybridization

reported in scientific literature (PMID 27133471)

Simple Western

1:50

Single Cell Western

1:10

Western Blot

2 ug/mL. Use reported in scientific literature (PMID 31082627)
Application Notes
In WB a specific band is observed ~160 kDa and an apparant non-specific band is observed ~56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human samples with WB. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 12 reviews rated 4.5 using NB110-89474 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD11b/Integrin alpha M

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). The CD11b/CD18 (Mac-1) heterodimer has a theoretical molecular weight of 127kDa but is often observed near 170kDa due to post translational modifications. Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix. Integrins have diverse roles in several biological processes, including cell migration during development, wound healing, cell differentiation, and apoptosis. The particular integrin CD11b is known as a pro-inflammatory molecule given its ability to promote phagocyte cytotoxic functions while enhancing several effector molecules such as FcGR, uPAR, and CD14 (1). In macrophages, CpG-DNA stimulates lysosomal degradation and vacuolar acidification, subsequently promoting CD11b release via TLR9 (2). During an acute infection, high CD11b/Mac-1 expression on antigen-specific CD8 (+) T cells can signify recent activation of the immune system, whereas naive T cells and virus-specific memory CD8 (+) T cells express little or no Mac-1 when exposed to a virus (3). Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder (4).

References

1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373

2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S., . . . Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677

3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593

4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S., . . . Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71

Alternate Names

CD11b, Integrin alpha M, ITGAM, MAC 1, MAC1, Mac1, alpha subunit, Mac-1a, Mo1, alpha subunit

Entrez Gene IDs

3684 (Human); 16409 (Mouse); 25021 (Rat)

Gene Symbol

ITGAM

UniProt

P05555 (Mouse), P11215 (Rat)

Additional CD11b/Integrin alpha M Products

Product Documents for CD11b Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD11b Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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