CD11c Antibody (AP-MAB0806) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-97871
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Armenian Hamster IgG Clone # AP-MAB0806
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This CD11c antibody was produced from a hybridoma (mouse myeloma fused with spleen cells) from an Armenian Hamster immunized with mouse lymphocytes.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 30455684).
Marker
Dendritic Cell Marker
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG
Scientific Data Images for CD11c Antibody (AP-MAB0806) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) - Azide and BSA Free [NB110-97871]
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Mouse spleen tissue.Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) - Azide and BSA Free [NB110-97871]
Immunohistochemistry-Paraffin: CD11c Antibody (AP-MAB0806) [NB110-97871] - Negative control, mouse spleen tissue section.Applications for CD11c Antibody (AP-MAB0806) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100-1:1000
Immunohistochemistry
1:25-1:100
Immunohistochemistry-Paraffin
1:25-1:100
Immunoprecipitation
1:100
Application Notes
4% PFA fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse CD11c. The antigen retrieval recommended for this antibody is the treatment with 20ug/ml Proteinase K in PBS for 15-25 minutes at room temperature. This antibody is CyTOF ready. Use in ICC/IF reported in scientific literature (PMID: 30300579). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31138656).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS.
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CD11c
Alternate Names
CD11c, Integrin alpha X, ITGAX, p150,95 alpha
Entrez Gene IDs
16411 (Mouse)
Gene Symbol
ITGAX
UniProt
Additional CD11c Products
Product Documents for CD11c Antibody (AP-MAB0806) - Azide and BSA Free
Product Specific Notices for CD11c Antibody (AP-MAB0806) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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