CD11c Antibody (BLR138H)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14718
Recombinant Monoclonal
![Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718]](https://resources.bio-techne.com/images/products/CD11c-Antibody-BLR138H-Immunohistochemistry-NBP3-14718-img0002.jpg "Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718]")
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Multiplex Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR138H
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
residues 112-355 (ECD)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for CD11c Antibody (BLR138H)
Detection of CD11c in Human Tonsil via seqIF™ Staining on COMET™
CD11c was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD11c Monoclonal Antibody (Catalog # NBP3-14718) at 1:80 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.![Western Blot: CD11c Antibody (BLR138H) [NBP3-14718]](https://resources.bio-techne.com/images/products/CD11c-Antibody-BLR138H-Western-Blot-NBP3-14718-img0001.jpg "Western Blot: CD11c Antibody (BLR138H) [NBP3-14718]")
Western Blot: CD11c Antibody (BLR138H) [NBP3-14718]
Western Blot: CD11c Antibody (BLR138H) [NBP3-14718] - Whole cell lysate (50 ug) from THP-1, HeLa, HEK293T, KG-1, Jurkat, Hep-G2, MCF-7, and NK-92 cells prepared using NETN lysis buffer. Rabbit anti-CD11c recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.![Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718]](https://resources.bio-techne.com/images/products/CD11c-Antibody-BLR138H-Immunohistochemistry-NBP3-14718-img0003.jpg "Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718]")
Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718]
Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718] - Rabbit anti-CD11c recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.Applications for CD11c Antibody (BLR138H)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 to 1:500
Immunohistochemistry-Paraffin
1:100 to 1:500
Multiplex Immunofluorescence
1:80
Western Blot
1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: CD11c
Alternate Names
CD11c, Integrin alpha X, ITGAX, p150,95 alpha
Gene Symbol
ITGAX
Additional CD11c Products
Product Documents for CD11c Antibody (BLR138H)
Product Specific Notices for CD11c Antibody (BLR138H)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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