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CD133 Antibody (3F10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37741

Novus Biologicals, part of Bio-Techne
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NBP2-37741
NBP2-37741SS

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F10

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Synthesized peptide of human CD133.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33814238).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD133 Antibody (3F10) - BSA Free

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human esophageal cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.
Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human breast cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.
Flow Cytometry: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Flow Cytometry: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Flow Cytometry: CD133 Antibody (3F10) [NBP2-37741] - Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).

Applications for CD133 Antibody (3F10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30739275).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (3F10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD133 Antibody (3F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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