CD133 Antibody (CL7971)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21163

Novus Biologicals, part of Bio-Techne
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NBP3-21163-100ul
NBP3-21163-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL7971

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD133 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Synthetic Peptide of O43490. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD133 Antibody (CL7971)

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human endometrium shows moderate positivity in apical membrane in glandular cells.
Western Blot: CD133 Antibody [NBP3-21163] -

Western Blot: CD133 Antibody [NBP3-21163] -

Analysis in human cell line NTERA-2.
View 4 more images

Applications for CD133 Antibody (CL7971)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (CL7971)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD133 Antibody (CL7971)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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