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CD133 Antibody (HL1271) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25403

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25403

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1271

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CD133 Antibody (HL1271) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the center region of Human CD133. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD133 Antibody (HL1271) - Azide and BSA Free

CD133 Antibody (HL1271) - Azide and BSA Free

Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] -

Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
CD133 Antibody (HL1271) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] -

Immunohistochemistry-Paraffin: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CD133 stained by CD133 antibody [HL1271] (NBP3-25403) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CD133 Antibody (HL1271) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] -

Immunohistochemistry-Paraffin: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CD133 stained by CD133 antibody [HL1271] (NBP3-25403) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for CD133 Antibody (HL1271) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (HL1271) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD133 Antibody (HL1271) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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