CD133 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44250

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CD133 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the mouse CD133 protein (between residues 650-750) [UniProt O54990]

Reactivity Notes

This antibody is not expected to react with human due to low homology.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD133 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NBP2-44250]

Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NBP2-44250]

Immunocytochemistry/Immunofluorescence: CD133 Antibody [NBP2-44250] - NIH 3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-mCD133 (NBP2-44250) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: CD133 AntibodyBSA Free [NBP2-44250]

Western Blot: CD133 AntibodyBSA Free [NBP2-44250]

Western Blot: CD133 Antibody [NBP2-44250] - Total mouse liver protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/mL anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Applications for CD133 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

2 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 30% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD133 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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