CD133 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59787

Novus Biologicals, part of Bio-Techne
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NBP2-59787
NBP2-59787-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD133 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acid sequence: QYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD133 Antibody

Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining in human placenta and skeletal muscle tissues.. Corresponding PROM1 RNA-seq data are presented for the same tissues.
Western Blot: CD133 Antibody [NBP2-59787]

Western Blot: CD133 Antibody [NBP2-59787]

Western Blot: CD133 Antibody [NBP2-59787] - Analysis in human cell line CACO-2 and human cell line HEK 293.
Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining of human placenta shows strong membranous positivity in decidual cells.
View 3 more images

Applications for CD133 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD133 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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