CD14 Antibody (1H5D8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37295

Novus Biologicals, part of Bio-Techne
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NBP2-37295
NBP2-37295-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1H5D8

Format

BSA Free

Concentration

1 mg/ml

View all CD14 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD14 Antibody (1H5D8) - BSA Free

Western Blot: CD14 Antibody (1H5D8)BSA Free [NBP2-37295]

Western Blot: CD14 Antibody (1H5D8)BSA Free [NBP2-37295]

Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: CD14 Antibody (1H5D8) - BSA Free [NBP2-37295]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (1H5D8) - BSA Free [NBP2-37295]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of HepG2 cells using CD14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: CD14 Antibody (1H5D8) - BSA Free [NBP2-37295]

Immunohistochemistry-Paraffin: CD14 Antibody (1H5D8) - BSA Free [NBP2-37295]

Immunohistochemistry-Paraffin: CD14 Antibody (1H5D8) [NBP2-37295] - Human tonsil FFPE tissue. Antigen retrieval with EDTA buffer. Image from verified customer review.
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Applications for CD14 Antibody (1H5D8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-37295 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (1H5D8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD14 Antibody (1H5D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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