CD14 Antibody (CL1637) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44005

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1637

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD14 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of D6RFL4, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD14 Antibody (CL1637) - Azide and BSA Free

CD14 Antibody (CL1637) - Azide and BSA Free Immunohistochemistry-Paraffin: CD14 Antibody [NBP3-44005]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP3-44005]

Analysis in human liver and pancreas tissues using NBP3-44005 antibody. Corresponding CD14 RNA-seq data are presented for the same tissues.
CD14 Antibody (CL1637) - Azide and BSA Free Western Blot: CD14 Antibody [NBP3-44005]

Western Blot: CD14 Antibody [NBP3-44005]

Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
CD14 Antibody (CL1637) - Azide and BSA Free Immunohistochemistry-Paraffin: CD14 Antibody [NBP3-44005]

Immunohistochemistry-Paraffin: CD14 Antibody [NBP3-44005]

Staining of human liver shows moderate to strong membranous positivity in Kupffer cells.
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Applications for CD14 Antibody (CL1637) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (CL1637) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD14 Antibody (CL1637) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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