Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow (Cell Surface), Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RPA-M1

Concentration

0.5 mg/ml

View all CD14 Antibodies »

Product Summary for CD14 Antibody (RPA-M1)

Immunogen

Human PHA-stimulated PBMC were used as immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD14 Antibody (RPA-M1)

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194]

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194]

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Analysis of CD14 in human PBMCs using anti-CD14 DyLight 405 conjugated antibody. Image from verified customer review. Image using the Azide Free format of this antibody.
Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194]

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194]

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Cell surface analysis of CD14 in 10^6 human monocytes using 0.1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD14 antibody. cell surface flow kit and anti-mouse IgG-FITC secondary was used for this test.
Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194]

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194]

Flow Cytometry: CD14 Antibody (RPA-M1) [NBP2-25194] - Cell surface analysis of CD14 PerCP/Cy5.5 in 10^6 human monocytes using 10 ul of this antibody. Green represents PerCP-Cy5.5 conjugated isotype control; red represents anti-CD14 antibody. Cell surface flow kit was used for this test.
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Applications for CD14 Antibody (RPA-M1)

Application
Recommended Usage

Flow (Cell Surface)

0.1-0.2 ug/10^6 cells

Flow Cytometry

0.1-0.2 ug/10^6 cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

CD14 antibody at 0.5 mg/ml in PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 a well known cell-activating receptor for lipopolysaccharide-binding protein and peptidoglycan is an important regulator of lipopolysaccharide (LPS)-dependent signal transduction and is a part of the multi-protein complex containing at least CD14, MD-2 and TLR4. Encoded by a single-copy gene, CD14 contains 11 LRR (leucine-rich) repeats and exists in two forms a glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. The soluble form of CD14 functions in LPS sensitivity to cells lacking the membrane form and its increased expression is reported to be associated with inflammatory infectious diseases and high mortality in gram-negative shock. CD14 an important component of the innate immune response preferentially expressed on mature cells of the monocytic lineage mediates the bacterial lipopolysaccharide (LPS) signaling, thus leading to NF-kappa-B activation, cytokine secretion and inflammatory response. Moreover it is also known to be involved in the clearance of apoptotic cells, up-regulation of cell surface molecules (adhesion molecules) and also in regulating IgE levels.

Alternate Names

CD14

Gene Symbol

CD14

UniProt

NP_000582

Additional CD14 Products

Product Documents for CD14 Antibody (RPA-M1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD14 Antibody (RPA-M1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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