CD14 Antibody (TUK4)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-62994

Clone TUK4 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # TUK4

Concentration

1 mg/ml

View all CD14 Antibodies »

Product Specifications

Immunogen

Made to Human CD14

Reactivity Notes

Predicted cross-reactivities: Rabbit, Porcine, Feline, Cynomolgus monkey, Dog, Bovine, Llama, Mink, Goat, Sheep

Localization

Cell Membrane

Specificity

This product recognises the human CD14 cell surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes. Clone Tuk4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner(6).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CD14 Antibody (TUK4)

Flow Cytometry: CD14 Antibody (TUK4) - BSA Free [NB100-62994]

Flow Cytometry: CD14 Antibody (TUK4) - BSA Free [NB100-62994]

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.
Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] Staining of human peripheral blood monocytes with Mouse anti Human CD14
Flow Cytometry: CD14 Antibody (TUK4) - BSA Free [NB100-62994]

Flow Cytometry: CD14 Antibody (TUK4) - BSA Free [NB100-62994]

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood granulocytes with Mouse anti Human CD14.
View 5 more images

Applications for CD14 Antibody (TUK4)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Alternate Names

CD14

Entrez Gene IDs

929 (Human)

Gene Symbol

CD14

UniProt

P08571

Additional CD14 Products

Product Documents for CD14 Antibody (TUK4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD14 Antibody (TUK4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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