CD14 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2807
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KRVDADADPRQYAD corresponding to internal region according to NP_000582.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for CD14 Antibody
Western Blot: CD14 Antibody [NB100-2807]
Western Blot: CD14 Antibody [NB100-2807] - Staining (1ug/ml) of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.Flow Cytometry: CD14 Antibody [NB100-2807]
Flow Cytometry: CD14 Antibody [NB100-2807] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Immunohistochemistry: Goat Polyclonal CD14 Antibody [NB100-2807] -
Immunohistochemistry: Goat Polyclonal CD14 Antibody [NB100-2807] - (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for CD14 Antibody
Application
Recommended Usage
ELISA
Detection limit 1:128000
Flow Cytometry
10 ug/ml
Immunohistochemistry
6-8 ug/ml
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx 55kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to the glycosylated form. Primary incubation 1 hour at room temperature. ICC/IF reactivity reported in (PMID: 25780044).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD14
Additional CD14 Products
Product Documents for CD14 Antibody
Product Specific Notices for CD14 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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