CD155/PVR Antibody (BLR074G)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78711

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-78711
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR074G

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD155/PVR Antibodies »

Product Summary for CD155/PVR Antibody (BLR074G)

Immunogen

A recombinant protein representing the extracellular domain of human PVR/CD155.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD155/PVR Antibody (BLR074G)

Western Blot: CD155/PVR Antibody (BLR074G) [NBP2-78711]

Western Blot: CD155/PVR Antibody (BLR074G) [NBP2-78711]

Western Blot: CD155/PVR Antibody (BLR074G) [NBP2-78711] - Detection of human PVR/CD155 by WB of 786-O, K-562, OVCAR-4, Jurkat, KM12, NCI-H226, RPMI-8226, and NCC-IT lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).
Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (BLR074G) [NBP2-78711]

Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (BLR074G) [NBP2-78711]

Immunocytochemistry/Immunofluorescence: CD155/PVR Antibody (BLR074G) [NBP2-78711] - Detection of human PVR/CD155 in FFPE HT29 cells by ICC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.
Immunohistochemistry-Paraffin: CD155/PVR Antibody (BLR074G) [NBP2-78711]

Immunohistochemistry-Paraffin: CD155/PVR Antibody (BLR074G) [NBP2-78711]

Immunohistochemistry-Paraffin: CD155/PVR Antibody (BLR074G) [NBP2-78711] - Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody [BLR074G]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: Opal™. Counterstain: DAPI.
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Applications for CD155/PVR Antibody (BLR074G)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:1000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. IHC and ICC/IF: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD155/PVR

Polio Virus Receptor (PVR) is a member of Ig-superfamily with 3 Ig-domains in arrangement V-C-C. Four mRNAs are produced from single gene for PVR, (mapped to human chromosome band 19q13.1-13.2). Two of the mRNAs (called H20A and H20B or alpha and delta) encode cell surface molecules of about 43kDa and 45kDa polypeptide backbone, respectively. Two other splice variants, beta and gamma encode secreted molecules of about 44kDa. The cytoplasmic tails are short (aa 35-50) and are rich in serine phosphorylated residues.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Gene Symbol

PVR

Additional CD155/PVR Products

Product Documents for CD155/PVR Antibody (BLR074G)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD155/PVR Antibody (BLR074G)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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