CD155/PVR Antibody (JE45-53)

Western Blot: CD155/PVR Antibody (JE45-53) [NBP3-32840]

Western Blot: CD155/PVR Antibody (JE45-53) [NBP3-32840] - Western blot analysis of CD155/PVR on different lysates with Rabbit anti-CD155/PVR antibody (NBP3-32840) at 1/1,000 dilution.

Lane 1: HUVEC cell lysate
Lane 2: U87-MG cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 45 kDa
Observed band size: 75 kDa

Exposure time: 4 minutes 39 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32840) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (JE45-53) [NBP3-32840]

Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (JE45-53) [NBP3-32840] - Immunocytochemistry analysis of HUVEC cells labeling CD155/PVR with Rabbit anti-CD155/PVR antibody (NBP3-32840) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD155/PVR antibody (NBP3-32840) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.