CD161/NK1.1 Antibody (PK-136) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20164CL1

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Cell depletion, Flow Cytometry

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # PK-136

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD161/NK1.1 Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing (C3H X BALB)F1 mice with spleen cells enriched for NK-1 cells and bone marrow cells from CE mice.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for CD161/NK1.1 Antibody (PK-136) [CoraFluor™ 1]

Application
Recommended Usage

Cell depletion

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Gene Symbol

Klrb1c

Additional CD161/NK1.1 Products

Product Documents for CD161/NK1.1 Antibody (PK-136) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD161/NK1.1 Antibody (PK-136) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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