CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80631

Novus Biologicals, part of Bio-Techne
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NBP2-80631
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Catalog #
BSA Free
NB100-77528
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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PK136

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD161/NK1.1 Antibodies »

Product Specifications

Immunogen

NK-1+ cells from mouse spleen and bone marrow

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631]

Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631]

Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Mouse. Image from the standard format of this antibody.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631]

Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631]

Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Analysis using the PE/Cy7 conjugate of NB100-77528. Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC and staining buffer (autofluorescence) (left) or Anti-Mouse NK1.1 PE-Cy7 (right). Total viable cells were used for analysis.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631]

Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631]

Flow Cytometry: CD161/NK1.1 Antibody (PK136) - Azide and BSA Free [NBP2-80631] - Analysis using the PerCP/Cy5.5 conjugate of NB100-77528. Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC and 0.25 ug of Mouse IgG2a K Isotype Control PerCP-Cy5.5 (left) or 0.25 ug of Anti-Mouse NK1.1 PerCP-Cy5.5 (right). Total viable cells were used for analysis.
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Applications for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1.0 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1:20-1:1000

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Frozen

1:20-1:1000

Immunohistochemistry-Paraffin

1:20-1:1000. Use reported in scientific literature (PMID 21118981)

Immunoprecipitation

1:20-1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 0.25 ug/1 x10^6 cells in 100 ul It is recommended that the reagent be titrated for optimal performance for each application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Gene Symbol

Klrb1c

Additional CD161/NK1.1 Products

Product Documents for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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