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CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08857

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PK136

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

CD Mouse spleen and bone marrow cells enriched for NK-1+ cells

Reactivity Notes

Does not react with Human.

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07532).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Gene Symbol

Klrb1c

Additional CD161/NK1.1 Products

Product Documents for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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