CD163 Antibody (K20-T)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30148

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP1-30148-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # K20-T

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD163 Antibodies »

Product Specifications

Immunogen

Peptide derived from N-terminal sequence of human CD163. Antibody recognizes the epitope between Gly134 - Gly148.

Epitope

Gly134 - Gly148

Specificity

This antibody is specific for the N terminus of CD163.

Marker

Histiocytic Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for CD163 Antibody (K20-T)

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163 immunolabelling on a FFPE tissue section of human HCV liver biopsy. Image provided by Victoria Gadd.
Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163 expression in the macrophages of the bone marrow. FFPE human tissues (4 um sections) stained with anti-CD163.
Immunocytochemistry/ Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148]

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148]

Immunocytochemistry/Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148] - Human primary macrophages in 3D cell culture. Primary antibodies to CD163 (NBP1-30148; RED) and CD206 (clone 15-2; GREEN) were both diluted at 1:100 in blocking buffer and incubated overnight at 4C. This image represents a 100-micron Z-Stack displayed as maximum intensity projection. Image from verified customer review.
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Applications for CD163 Antibody (K20-T)

Application
Recommended Usage

Immunohistochemistry

1:100 -1:200

Immunohistochemistry-Paraffin

1:100 - 1:200
Application Notes
Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 24798518).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-30148 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris-HCl (pH 8.0) and 20mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD163

CD163 (Cluster of Differentiation 163), also known by several other names including M130, p155, RM3/1, Ki-M8, Ber-MAC3, SM4, and GHI/61, is a type I transmembrane glycoprotein that is a member of the scavenger receptor cysteine-rich (SRCR) super family class B (1-3). CD163 is expressed specifically on the monocyte/macrophage lineage and has a theoretical molecular weight of 130-160 kDa in reducing conditions and 110 kDa in non-reducing conditions (2 - 6). CD163 is synthesized as 1076 amino acid (aa) protein consisting of a large extracellular domain (ECD) with nine SRCR domains, proline-serine-threonine rich (PST) linker domain, a transmembrane segment, and a cytoplasmic tail which has varying lengths depending on the isoform due to alternative splicing, with the short 49 aa form being the most common (1-3, 6).

One of the primary functions of CD163 is uptake of haptoglobin-hemoglobin (Hp-Hb) complexes from the liver, spleen, and bone marrow, ultimately triggering an anti-inflammatory response (3, 5, 7). CD163 also functions as an erythroblast adhesion receptor and promotes cell maturation and survival (3, 5, 7). Furthermore, CD163 functions in immune sensing of bacteria and as a receptor for tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) (3, 5, 7). As mentioned above, CD163 is expressed on cells in the monocyte/macrophage lineage and, in general, anti-inflammatory signals including glucocorticoids, interleukin (IL)-6, and IL-10 stimulate CD163 synthesis and expression while, conversely, pro-inflammatory signals such as interferon-gamma (INF-gamma), TNF-alpha, and lipopolysaccharide (LPS) downregulate CD163 (3, 5). In addition to membrane-bound form of CD163, the protein can be cleaved by metalloproteinases (MMP) and induced by LPS or phorbol myristate acetate (PMA) to release a soluble form (sCD163) into the plasma (7). Increased levels of sCD163 in the plasma and an increased number of CD163-expressing macrophages at the site of inflammation are associated with a variety of pathologies (3, 5-7). CD163/sCD163 is often increased and a suitable clinical marker for inflammatory diseases including rheumatoid arthritis (RA), Gaucher disease, chronic kidney disease, diabetes, and Crohn's disease (3, 5-7).

Alternative names for CD163 includes GHI/61, HbSR, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, RM3/1, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, sCD163, and soluble CD163.

References

1. Law, S. K., Micklem, K. J., Shaw, J. M., Zhang, X. P., Dong, Y., Willis, A. C., & Mason, D. Y. (1993). A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. European journal of immunology. https://doi.org/10.1002/eji.1830230940

2. Onofre, G., Kolackova, M., Jankovicova, K., & Krejsek, J. (2009). Scavenger receptor CD163 and its biological functions. Acta medica (Hradec Kralove).

3. Van Gorp, H., Delputte, P. L., & Nauwynck, H. J. (2010). Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy. Molecular immunology. https://doi.org/10.1016/j.molimm.2010.02.008

4. Sulahian, T. H., Hogger, P., Wahner, A. E., Wardwell, K., Goulding, N. J., Sorg, C., Droste, A., Stehling, M., Wallace, P. K., Morganelli, P. M., & Guyre, P. M. (2000). Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. https://doi.org/10.1006/cyto.2000.0720

5. Etzerodt, A., & Moestrup, S. K. (2013). CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxidants & redox signaling. https://doi.org/10.1089/ars.2012.4834

6. Skytthe, M. K., Graversen, J. H., & Moestrup, S. K. (2020). Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases. International journal of molecular sciences, 21(15), 5497. https://doi.org/10.3390/ijms21155497

7. Moller H. J. (2012). Soluble CD163. Scandinavian journal of clinical and laboratory investigation. https://doi.org/10.3109/00365513.2011.626868

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Entrez Gene IDs

9332 (Human)

Gene Symbol

CD163

UniProt

Q86VB7

Additional CD163 Products

Product Documents for CD163 Antibody (K20-T)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD163 Antibody (K20-T)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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