CD1a Antibody (O10) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45341

Clone O10 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-45341
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Key Product Details

Species Reactivity

Human, Rhesus Macaque

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # O10

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD1a Antibodies »

Product Specifications

Immunogen

Human thymus cells

Localization

Cell surface

Marker

Mature Langerhans Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for CD1a Antibody (O10) - IHC-Prediluted

Immunohistochemistry: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341]

Immunohistochemistry: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341]

Immunohistochemistry: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341] - Human skin. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.
Immunohistochemistry-Paraffin: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341]

Immunohistochemistry-Paraffin: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341]

Immunohistochemistry-Paraffin: CD1a Antibody (O10) - IHC-Prediluted [NBP2-45341] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10).

Applications for CD1a Antibody (O10) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD1a

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Long Name

T Cell Surface Glycoprotein CD1a

Alternate Names

CD1a, FCB6, HTA1, R4, T6

Gene Symbol

CD1A

Additional CD1a Products

Product Documents for CD1a Antibody (O10) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD1a Antibody (O10) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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