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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-2

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Rat thymocyte membrane glycoproteins

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65320 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells. Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation. This product is routinely tested by flow cytometry on rat thymocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD200/OX2 Antibody (OX-2)

Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]

Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]

Flow Cytometry: CD200/OX2 Antibody (OX-2) [NB100-65320] - Analysis of PE conjugate of NB100-65320. Staining of rat thymocytes with Mouse anti Rat CD200
Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]

Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]

Flow Cytometry: CD200/OX2 Antibody (OX-2) [NB100-65320] - Staining of rat thymocytes with Mouse anti Rat CD200/OX2.

Applications for CD200/OX2 Antibody (OX-2)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD200

CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. It is a monomorphic determinant expressed on thymocytes, brain, follicular dendritic cells in all lymphoid organs, endothelium and at low levels on some smooth muscle and B lymphocytes. Recent studies have suggested that the CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.

Alternate Names

CD200, MOX1, MOX2, MRC, OX-2

Entrez Gene IDs

24560 (Rat)

Gene Symbol

CD200

UniProt

Additional CD200 Products

Product Documents for CD200/OX2 Antibody (OX-2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD200/OX2 Antibody (OX-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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