CD21/CD35 Antibody (7E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11263

Novus Biologicals, part of Bio-Techne
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NBP3-11263
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 7E9

Concentration

0.1 mg/ml

View all CD21/CD35 Antibodies »

Product Specifications

Immunogen

Purified mouse CR1 from serum

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for CD21/CD35 Antibody (7E9)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Flow Cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD21/CD35

The monoclonal antibody 7E9 recognizes mouse complement receptors type 1 (CR1) and 2 (CR2). CR1 and CR2 are cell surface glycoproteins that are capable of binding to activation fragments of the third and/or fourth complement components (C3 and/or C4). They play a role in the clearance of immune-complexes, phagocytosis, complement regulation, and immunoregulation. Mouse CR1 (MCR1, 190 kD) is found on the surface of B-lymphocytes, follicular dendritic cells and at lower levels on peritoneal macrophages and activated granulocytes. MRC1 has binding activity for C3b and serves as a cofactor for factor I-mediated cleavage of C3b. Mouse CR2 (MCR2, 150 kD) is a type I transmembrane glycoprotein that binds complement fragments (C3d(g), iC3b) and interferon (IFN)-alpha. MCR2 is expressed on B-lymphocytes and probably on follicular dendritic cells. On human B lymphocytes it acts as the Epstein-Barr virus (EBV) receptor. MCR2 mediates the formation of rosettes between B-lymphocytes and E-bearing Crd. MCR1 and MRC2 are very closely related. They are both products of a single gene, Cr2, formed by alternative splicing of mRNA. MCR2 corresponds to the carboxy-terminal portion of MCR1. This is in contrast with human CR1 (CD35) and CR2. The monoclonal antibody 7E9 does not inhibit rosette formation between 2PK3 cells and Crd-coated SRBC indicator cells.

Alternate Names

C3b/C4b receptor, C3-binding protein, C3BR, C3DR, C4BR, CD35, CD35 antigen, complement component (3b/4b) receptor 1 (Knops blood group), complement component (3d/Epstein Barr virus) receptor 2, complement component 3d receptor 2, complement receptor 1, complement receptor type 1, complement receptor type 2, CR, CVID7, EBV receptor, KN, Knops blood group antigen, SLEB9

Gene Symbol

CR2

Additional CD21/CD35 Products

Product Documents for CD21/CD35 Antibody (7E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD21/CD35 Antibody (7E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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