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CD24 Antibody (SN3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64861

Clone SN3 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-64861
NB100-64861-0.025mg

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SN3

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Glycoproteins purified from human NALM-1 cell line

Reactivity Notes

Predicted cross-reactivities: Chimpanzee

Specificity

Recognizes the human CD24 cell surface antigen, a heavily glycosylated molecule of 35-45kD. CD24 is expressed by granulocytes and B lymphocytes, and by some activated T cells and T cell malignancies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD24 Antibody (SN3) - BSA Free

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861] - SUM159PT cells were stained with CD24 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: CD24 antibody stain. Image from verified customer review.
Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) - BSA Free [NB100-64861]

Flow Cytometry: CD24 Antibody (SN3) [NB100-64861] - Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD24: RPE.

Applications for CD24 Antibody (SN3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1/50-1/100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
This CD24 antibody is validated for IHC-P from a verified customer review.

Reviewed Applications

Read 1 review rated 3 using NB100-64861 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD24

CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer.

Alternate Names

CD24, CD24A

Gene Symbol

CD24

UniProt

Additional CD24 Products

Product Documents for CD24 Antibody (SN3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD24 Antibody (SN3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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