CD25/IL-2R alpha Antibody (216)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89307

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 216

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD25/IL-2R alpha Antibodies »

Product Summary for CD25/IL-2R alpha Antibody (216)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD25/IL-2R alpha (Accession#: NP_000408.1; Met1-Cys213).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD25/IL-2R alpha Antibody (216)

Flow Cytometry: CD25/IL-2R alpha Antibody (216) [NBP2-89307]

Flow Cytometry: CD25/IL-2R alpha Antibody (216) [NBP2-89307]

Flow Cytometry: CD25/IL-2R alpha Antibody (216) [NBP2-89307] - Analysis of IL2RA(CD25) expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin, then stained with purified anti-Human IL2RA(CD25), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.

Applications for CD25/IL-2R alpha Antibody (216)

Application
Recommended Usage

Flow Cytometry

1:25-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (216)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD25/IL-2R alpha Antibody (216)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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