Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 745520

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD25/IL-2R alpha Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant rat CD25/IL-2 R alpha
Glu22-Gln235
Accession # P26897

Specificity

Detects rat CD25/IL-2 R alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) CD25/IL-2 R alpha, rmIL-2 R beta, rmCommon gamma chain, rmIL-15 R alpha, or recombinant human CD25/IL-2 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD25/IL-2R alpha Antibody (745520) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (745520) [PE/Cy7]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD25/IL-2R alpha Antibody (745520) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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