CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75902
![Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free [NBP2-75902]](https://resources.bio-techne.com/images/products/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Immunohistochemistry-NBP2-75902-img0001.jpg "Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free [NBP2-75902]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Cynomolgus Monkey, Rhesus Macaque
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Human IgG1 kappa Clone # Basiliximab
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
Specificity
Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit -binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ((alpha)(beta)(gamma)c) -T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Clonality
Monoclonal
Host
Human
Isotype
IgG1 kappa
Scientific Data Images
Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free [NBP2-75902]
Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formalin fixed rat spleen slices were were stained with a NBP2-75902 at 5 ug/ml. The anti-human CD25 antibody NBP2-75902 also shows some cross-reactivity with rat in IHC.
Immunocytochemistry/Immunofluorescence: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] -
Immunocytochemistry/Immunofluorescence: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formaldehyde-fixed rat thymus slices were stained with NBP2-75902 at 5 ug/ml and detected with a FITC-conjugated secondary antibody. Ab00188 shows weak labelling of rat cells by IF.Applications
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA, biosimilar antibody, biosimilars
Gene Symbol
IL2RA
Additional CD25/IL-2R alpha Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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