Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # PC61.5.3

Format

BSA Free

Concentration

1.0 mg/ml

View all CD25/IL-2R alpha Antibodies »

Product Specifications

Immunogen

B6.1 CTL cell line.

Localization

Cell Membrane, Cytoplasm

Specificity

This reacts with the low affinity alpha chain of the IL2 receptor present on activated T and B cells in mice. Clone PC61.5.3 is reported to inhibit IL2 binding and IL2 dependent proliferation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for CD25/IL-2R alpha Antibody (PC61.5.3) - BSA Free

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (PC61.5.3) - BSA Free [NB600-710]

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (PC61.5.3) - BSA Free [NB600-710]

Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (PC61.5.3) [NB600-710] - Analysis of formalin-fixed paraffin-embedded tissue section of spleen from normal Mouse using CD25/IL-2 R alpha antibody (clone PC61.5.3.) at 1:100 dilution.

Applications for CD25/IL-2R alpha Antibody (PC61.5.3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Flow Cytometry: Use 50ul of the suggested working dilution to label 1x10sup6/sup cells in 100ul. Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for CD25/IL-2R alpha Antibody (PC61.5.3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD25/IL-2R alpha Antibody (PC61.5.3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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