CD27 Ligand/TNFSF7/CD70 Antibody (BU69) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11586
Clone BU69 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BU69
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human WM-1 (Waldenstrom s macroglobulinemia) cell line
Localization
Cell Surface
Specificity
It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This monoclonal antibody blocks the interaction between CD27 and CD70, and has been shown to inhibit T cell proliferation induced by dendritic cells.
Marker
Activated T- & B-Lymphocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44545).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for CD27 Ligand/TNFSF7/CD70 Antibody (BU69) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Frozen
0.5 - 1 ug/ml
Immunoprecipitation
0.5 - 1 ug/500ug Protein Lysate
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Inhibits T cell proliferation induced by dendritic cells: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Inhibits T cell proliferation induced by dendritic cells: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD27 Ligand/TNFSF7
Alternate Names
CD70, TNFSF7
Gene Symbol
CD70
Additional CD27 Ligand/TNFSF7 Products
Product Documents for CD27 Ligand/TNFSF7/CD70 Antibody (BU69) - Azide and BSA Free
Product Specific Notices for CD27 Ligand/TNFSF7/CD70 Antibody (BU69) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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