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CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28627

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # cusatuzumab

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

TNFSF7 / CD27L / CD70

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa.

Also known as 'cusatuzumab'.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

ELISA: CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized[NBP3-28627] -

Immobilized huaman CD70 FC at 4 ug/mL can bind CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized, EC50=0.08279 ug/mL.
CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

Flow Cytometry: CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized[NBP3-28627] -

Human TNFSF7 / CD27L / CD70 CHO-K1 Cell Linewere stained with cusatuzumab and negative control protein respectively, washed and then followed by PE and analyzed with FACS,EC50=0.03451 ug/mL.
CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

Flow Cytometry: CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized[NBP3-28627] -

HL-60 cells were stained with CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.2727 ug/mL.

Applications for CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD27 Ligand/TNFSF7

CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.

Alternate Names

CD70, TNFSF7

Gene Symbol

CD70

UniProt

Additional CD27 Ligand/TNFSF7 Products

Product Documents for CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD27 Ligand/TNFSF7/CD70 Antibody (cusatuzumab) - Humanized

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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