CD3 delta Antibody (1F4)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65230

Novus Biologicals, part of Bio-Techne
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NB100-65230
NB100-65230-0.025mg
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Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 1F4

Concentration

1.0 mg/ml

View all CD3 delta/CD3d Antibodies »

Product Specifications

Immunogen

F344 rat T cells stimulated with PMA (TPA) and calcium ionophore

Localization

Type I membrane protein.

Specificity

Recognizes Rat CD3D (Gene ID: 25710, UniProt: P19377) and Rat CD3G (Gene ID: 300678, UniProt: Q64159) NB100-65230 recognizes rat CD3, a 25kD antigen which is found on rat T-cells. NB100-65230 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for CD3 delta Antibody (1F4)

Immunohistochemistry-Paraffin: CD3 delta Antibody (1F4) [NB100-65230]

Immunohistochemistry-Paraffin: CD3 delta Antibody (1F4) [NB100-65230]

Immunohistochemistry-Paraffin: CD3 Antibody (1F4) [NB100-65230] - Staining of paraffin embedded rat spleen with Mouse anti Rat CD3.

Applications for CD3 delta Antibody (1F4)

Application
Recommended Usage

Flow Cytometry

1:10-1:25

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:25

Immunohistochemistry-Paraffin

1:10

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 delta/CD3d

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Long Name

CD3 delta

Alternate Names

CD3D

Gene Symbol

CD3D

Additional CD3 delta/CD3d Products

Product Documents for CD3 delta Antibody (1F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 delta Antibody (1F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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