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CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52641AF647

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-52641AF647

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry, Functional, Immunoprecipitation, Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 145-2C11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric

Immunogen

H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone.

Reactivity Notes

Rat

Specificity

Recognises a 25-kDa protein component (CD3e, originally called T3-e) of the antigen-specific T-cell receptor.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric

CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric

CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric [NBP2-52641AF647] -

CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric [NBP2-52641AF647] - Vial of Alexa Fluor 647 conjugated antibody. Alexa Fluor 647 is optimally excited at 653 nm by the Red laser (633 or 640 nm) and has an emission maximum of 669 nm.

Applications for CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 647] - Chimeric



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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