CD3 epsilon Antibody (C3e/1308) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54392APC
![CD3 epsilon Antibody (C3e/1308) [Allophycocyanin]](https://resources.bio-techne.com/images/products/nbp2-54392apc_mouse-monoclonal-cd3-epsilon-antibody-c3e-1308-allophycocyanin-31720239483920.jpg "CD3 epsilon Antibody (C3e/1308) [Allophycocyanin] [NBP2-54392APC] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Multiplex Immunoassay, Western Blot
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # C3e/1308
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human CD3 epsilon fragment (aa 23-119) (Uniprot: P07766)
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 33428661).
Localization
Cell surface and Cytoplasmic
Specificity
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Scientific Data Images for CD3 epsilon Antibody (C3e/1308) [Allophycocyanin]
CD3 epsilon Antibody (C3e/1308) [Allophycocyanin] [NBP2-54392APC] -
CD3 epsilon Antibody (C3e/1308) [Allophycocyanin] [NBP2-54392APC] - Vial of APC conjugated antibody. APC is optimally excited at 650 nm by the Red laser (633 or 640 nm) and has an emission maximum of 660 nm.Applications for CD3 epsilon Antibody (C3e/1308) [Allophycocyanin]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Multiplex Immunoassay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
Optimal dilution of this antibody should be experimentally determined.
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD3 epsilon
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for CD3 epsilon Antibody (C3e/1308) [Allophycocyanin]
Product Specific Notices for CD3 epsilon Antibody (C3e/1308) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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