CD3 epsilon Antibody (CL1466) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43997

Novus Biologicals, part of Bio-Techne
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NBP3-43997
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BSA Free
NBP2-34483
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1466

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07766, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD3 epsilon Antibody (CL1466) - Azide and BSA Free

CD3 epsilon Antibody (CL1466) - Azide and BSA Free Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP3-43997]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP3-43997]

Analysis in human tonsil and skeletal muscle tissues using NBP3-43997 antibody. Corresponding CD3 epsilon RNA-seq data are presented for the same tissues.
CD3 epsilon Antibody (CL1466) - Azide and BSA Free Western Blot: CD3 epsilon Antibody [NBP3-43997]

Western Blot: CD3 epsilon Antibody [NBP3-43997]

Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
CD3 epsilon Antibody (CL1466) - Azide and BSA Free Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP3-43997]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP3-43997]

Staining of human fallopian tube shows moderate membranous positivity in lymphoid cells.
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Applications for CD3 epsilon Antibody (CL1466) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (CL1466) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 epsilon Antibody (CL1466) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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