Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 157 to 207 of human CD3e Molecule, Epsilon (CD3-TCR Complex) using the numbering given in entry NP_000724.1 (GeneID 916).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD3 epsilon Antibody - BSA Free

Western Blot: CD3 epsilon Antibody [NBP2-32282]

Western Blot: CD3 epsilon Antibody [NBP2-32282]

Western Blot: CD3 epsilon Antibody [NBP2-32282] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-32282]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-32282]

Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-32282] - Sample: FFPE section of human lung cancer infiltrate. Antibody: Affinity purified rabbit anti- CD3E used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: CD3 epsilon Antibody [NBP2-32282]

Immunoprecipitation: CD3 epsilon Antibody [NBP2-32282]

Immunoprecipitation: CD3 epsilon Antibody [NBP2-32282] - Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for IP at 6 ug per reaction. CD3E was also immunoprecipitated by rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (NBP2076424) and another rabbit anti-CD3E antibody. For blotting immunoprecipitated CD3E, NBP2-32282 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.

Applications for CD3 epsilon Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

UniProt

NP_000724.1 (Human)

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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